BEGIN:VCALENDAR VERSION:2.0 PRODID:researchseminars.org CALSCALE:GREGORIAN X-WR-CALNAME:researchseminars.org BEGIN:VEVENT SUMMARY:Christoph Allolio DTSTART:20251105T080000Z DTEND:20251105T090000Z DTSTAMP:20260422T122146Z UID:MathMAC/42 DESCRIPTION:Title: Molecular Grammar of Microtubule-Wetting Biomolecular Condensates\nby Christoph Allolio as part of Modelling of materials - theory\, model redu ction and efficient numerical methods (UNCE MathMAC)\n\n\nAbstract\nBiomol ecular condensates are a recently discovered new ordering principle of the cell. They consist of partially disordered proteins\, which undergo liqui d-liquid phase separation inside the cytosol\, turning the cell into a mul tiphase mixture. Microtubuli are the backbone of the cytoskeleton in the s ense that they are the entities inside the cell that is able to generate t he largest mechanical force.\n\nMicrotubule condensate interactions are fu ndamental for cell division\, vesicle transport and cellular locomotion. A ccordingly\, they represent a large number of attractive drug targets. Due to the size of microtubuli and the slow timescale of condensate structura l relaxation\, there has not been a systematic investigation at the molecu lar level as to what binding patterns (molecular grammar) enable condensat e binding to microtubuli. We provide a protocol that is able to predict wh ether any given disordered protein sequence will bind to microtubuli. This protocol is suitable for high-throughput screening. Our pattern analysis allows us to establish two categories of strongly interacting subsequences that enable binding to microtubuli: positively charged hydrophobic cluste rs and alternating charge sequences. Their overall optimal balance is anal yzed and preferential regions of interaction on microtubuli are identified and validated with known experimental results. Our results enable rapid p rototyping of proteins that target the microtubule surface\, i.e. they pre dict whether unstructured proteins will wet the microtubule interface.\n LOCATION:https://researchseminars.org/talk/MathMAC/42/ END:VEVENT END:VCALENDAR