BEGIN:VCALENDAR VERSION:2.0 PRODID:researchseminars.org CALSCALE:GREGORIAN X-WR-CALNAME:researchseminars.org BEGIN:VEVENT SUMMARY:Cristian Micheletti (International School for Advanced Studies (SI SSA)) DTSTART:20240108T210000Z DTEND:20240108T220000Z DTSTAMP:20260423T041459Z UID:GEOTOP-A/72 DESCRIPTION:Title: Dynamics and mechanics of knotted DNA and RNAs: insights from molecular dynamics simulations\nby Cristian Micheletti (International School for Advanced Studies (SISSA)) as part of GEOTOP-A seminar\n\n\nAbstract\nI wi ll report on a series of studies where we used molecular dynamics simulati ons and various models to study how the properties of DNA and RNAs are aff ected by the presence of knots and other forms of structural entanglement[ 1]. I will first consider model DNA plasmids that are both knotted and sup ercoiled\, and discuss how the simultaneous presence of knots and supercoi ling creates long-lived multi-strand interlockings that might may be relev ant for the simplifying action of topoisomerases. I next consider how enta ngled nucleic acids behave when driven through narrow pores[2-4]\, a setti ng that models translocation through the lumen of enzymes\, and discuss th e biological implication for a certain class of viral RNAs[4].\n\n \n[1] L . Coronel\, A. Suma and C. Micheletti\, "Dynamics of supercoiled DNA with complex knots"\, Nucleic Acids Res. (2018) 46 \, 7533 \n\n[2] A. Suma\, V. Carnevale and C. Micheletti\, Nonequilibrium thermodynamics of DNA nanopo re unzipping\, Phys. Rev. Lett.\, (2023)\, 130 048101\n\n[3] A. Suma\, A. Rosa and C. Micheletti\, Pore translocation of knotted polymer chains: how friction depends on knot complexity\, ACS Macro Letters\, (2015)\, 4 \, 1 420-1424\n\n[4] A. Suma\, L. Coronel\, G. Bussi and C. Micheletti\, "Direc tional translocation resistance of Zika xrRNA” Nature Communications (20 20)\, 11 \, art no. 3749\n LOCATION:https://researchseminars.org/talk/GEOTOP-A/72/ END:VEVENT END:VCALENDAR